Polycomb protein Suz12, Suz12
ELISA KIT/ Mouse Polycomb protein Suz12, Suz12 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-29985m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Polycomb protein Suz12, Suz12
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Polycomb protein Suz12, Suz12 ELISA KIT
Product Name:Mouse Polycomb protein Suz12, Suz12 ELISA KIT
Packing:96T
Catalog No.:ELI-29985m
Gene Name:Mouse Suz12
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Suz12
Alternative Name:Mouse Polycomb protein Suz12, Suz12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Polycomb protein Suz12, Suz12 ELISA KIT allows for the in vitro quantitative determination of Mouse Suz12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Polycomb protein Suz12, Suz12 ELISA KIT has been pre-coated with an Mouse Polycomb protein Suz12, Suz12 antibody specific to Mouse Suz12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Suz12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Suz12 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Suz12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验家公司的免疫沉淀试剂盒还是 SimpleChIP® 试剂盒，结果均是如此。在分析稳定性较低的相互作用，比如 polycomb 蛋白（Ezh2 [D] 或 SUZ12 [E]）与特定基因的结合时，这一点尤其明显。 第 3 步 - 选择抗体 非高度特异的靶标抗体可能会出现不可预见的结合并增加背景信号，从而增加检测低丰度或低稳定性相互作用的难度。 您的抗体应该满足以下标准： 靶标特异性： 抗体应与阳性/阴性对照细胞系、敲除细胞或经过 siRNA
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
) Antibody directed against protein of interest (e.g., Upstate Biotechnology, Santa Cruz Biotech, Abcam) Triton X‐100
所的科学家将从基因组学的水平分析H2AZ，新的研究结果表明H2AZ的作用模式与PcG（Polycomb group）蛋白Suz12惊人的相似，它们是某些重要的基因的启动子蛋白。 8. The Growth Factor Environment Defines Distinct Pluripotent Ground States in Novel Blastocyst-Derived Stem Cells 细胞具有多能性，这是一种维持细胞自我更新以及产生三胚层衍生组织的能力。长久以来科学
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