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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Sentrin-specific protease 5, Senp5
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Sentrin- specific protease 5, Senp5 ELISA KIT
Product Name:Mouse Sentrin- specific protease 5, Senp5 ELISA KIT
Packing:96T
Catalog No.:ELI-29715m
Gene Name:Mouse Senp5
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Senp5
Alternative Name:Mouse Sentrin-specific protease 5, Senp5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Sentrin- specific protease 5, Senp5 ELISA KIT allows for the in vitro quantitative determination of Mouse Senp5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Sentrin- specific protease 5, Senp5 ELISA KIT has been pre-coated with an Mouse Sentrin-specific protease 5, Senp5 antibody specific to Mouse Senp5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Senp5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Senp5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Senp5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
detailed insight in considerations to choose and set up the appropriate mouse model for specific vascular research questions. Additionally, we provide technical details to execute experiments with these animal models. Curr. Protoc. Mouse Biol. 2:317?345 ©
Chromosome Conformation Capture (3C) (PROT05)
in the nucleus. Novel ligation products can be detected by PCR. We adapted the 3C assay (Dekker et al ., 2002) to determine the conformation of mouse chromosome 7 and in particular the co-localization of actively transcribed genes in transcription factories
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