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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
40S ribosomal protein S21, Rps21
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse 40S ribosomal protein S21, Rps21 ELISA KIT
Product Name:Mouse 40S ribosomal protein S21, Rps21 ELISA KIT
Packing:96T
Catalog No.:ELI-29500m
Gene Name:Rps21
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse 40S ribosomal protein S21
Alternative Name:Rps21,Mouse 40S ribosomal protein S21
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 40S ribosomal protein S21, Rps21 ELISA KIT allows for the in vitro quantitative determination ofMouse 40S ribosomal protein S21 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 40S ribosomal protein S21, Rps21 ELISA KIT has been pre-coated with an Rps21 antibody specific to Mouse 40S ribosomal protein S21 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse 40S ribosomal protein S21 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse 40S ribosomal protein S21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse 40S ribosomal protein S21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验构成核糖体的蛋白质。大肠杆菌核糖体蛋白的初级结构均被确定。大肠杆菌核糖体的 30S亚基含 S1 — S21 共 21种蛋白质, 50S亚基含 L1— L34共 34种蛋白质。这些蛋白质已被全部分离纯化。分子量约 1万到 3万。除 S6 、 L7 、 L12 之外全是碱性蛋白质。这些蛋白质是免疫学上独立的蛋白质,只有 L7 、 L1 2 显示出相互交叉反应。已知 L7 与 L1 2 是同一蛋白质, L7 的 N末端被乙酰化。已经确定了几种蛋白的一级结构。机能已经
the binding sites for the 40S ribosomal subunit and for other translation initiation signals. Alternatively, the formation of a double-stranded DNA/RNA complex can render the RNA susceptible to RNase H digestion (2 ). The antigene approach is based
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
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