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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
GRB2-related adaptor protein 2, Grap2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse GRB2-related adaptor protein 2, GRAP2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GRAP2,GADS; GRAP-2; GRB2L; GRBLG; GRID; GRPL; GrbX; Grf40; Mona; P38; GRB-2-like protein; GRB2-related protein with insert domain; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule; adapter protein GRID; grf-40; grf40 adapter protein; growth factor receptor-binding protein; growth factor receptor-bound protein 2-related adaptor protein 2; hematopoietic cell-associated adapter protein GrpL; hematopoietic cell-associated adaptor protein GRPL; GRB2-related adaptor protein 2, GRB-2-related monocytic adapter protein,MONA,Monocytic adapter,GRB-2-like protein,GADS protein,
Search name
Mouse GRAP2 ELISA KIT ,Mouse GADS ELISA KIT ,Mouse GRAP-2 ELISA KIT ,Mouse GRB2L ELISA KIT ,Mouse GRBLG ELISA KIT ,Mouse GRID ELISA KIT ,Mouse GRPL ELISA KIT ,Mouse GrbX ELISA KIT ,Mouse Grf40 ELISA KIT ,Mouse Mona ELISA KIT ,Mouse P38 ELISA KIT ,Mouse GRB-2-like protein ELISA KIT ,Mouse GRB2-related protein with insert domain ELISA KIT ,Mouse SH3-SH2-SH3 adapter Mona ELISA KIT ,Mouse SH3-SH2-SH3 adaptor molecule ELISA KIT ,Mouse adapter protein GRID ELISA KIT ,Mouse grf-40 ELISA KIT ,Mouse grf40 adapter protein ELISA KIT ,Mouse growth factor receptor-binding protein ELISA KIT ,Mouse growth factor receptor-bound protein 2-related adaptor protein 2 ELISA KIT ,Mouse hematopoietic cell-associated adapter protein GrpL ELISA KIT ,Mouse hematopoietic cell-associated adaptor protein GRPL ELISA KIT ,Mouse GRB2-related adaptor protein 2 ELISA KIT ,Mouse GRB-2-related monocytic adapter protein ELISA KIT ,Mouse MONA ELISA KIT ,Mouse Monocytic adapter ELISA KIT ,Mouse GRB-2-like protein ELISA KIT ,Mouse GADS protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse grb2-related adaptor protein 2,GRAP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRAP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GRAP2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GRAP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GRAP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Mapping of Signaling Pathways by Functional Interaction Proteomics
is the differential analysis of binding partners of the C-terminal SH3 domain of the two small adaptor proteins Grb2 and GRAP.
Image Figure 2.12.5 Alignment of Grb2 protein sequences. Alignment of human, mouse, rat, worm, fly, and mosquito Grb2 sequences generated
, binding of PI3K to activated RAS can also stabilize its membrane localization and activate the catalytic domain. This occurs by recruitment of the adaptor proteins SHC, GRB2 and GAB2 to activated RTKs. C2, C2 domain; CD, catalytic domain; p85 BD, p85
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