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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein lin-7 homolog A, Lin7a
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein lin- 7 homolog A, Lin7a ELISA KIT
Product Name:Mouse Protein lin- 7 homolog A, Lin7a ELISA KIT
Packing:96T
Catalog No.:ELI-27641m
Gene Name:Mouse Lin7a
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Lin7a
Alternative Name:Mouse Protein lin-7 homolog A, Lin7a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein lin- 7 homolog A, Lin7a ELISA KIT allows for the in vitro quantitative determination of Mouse Lin7a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein lin- 7 homolog A, Lin7a ELISA KIT has been pre-coated with an Mouse Protein lin-7 homolog A, Lin7a antibody specific to Mouse Lin7a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lin7a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lin7a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lin7a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
) Antibody directed against protein of interest (e.g., Upstate Biotechnology, Santa Cruz Biotech, Abcam) Triton X‐100
bacteriophage and plasmid DNA. Nature468(7320):67–71. doi: 10.1038/nature09523 19. Cong L, Ran FA, Cox D, Lin S, Barretto R,Habib N, Hsu PD, Wu X, Jiang W, Marraffi niLA, Zhang F (2013) Multiplex genome engineeringusing CRISPR/Cas systems. Science339(6121
, Haverkamp TH, van Luenen HG, and Plasterk RH. (1999). Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNase D. Cell 99: 133-141. (Abstract) Grishok A, Pasquinelli AE, Conte D, Li N
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