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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Elongation factor G, mitochondrial, Gfm1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Elongation factor G, mitochondrial, GFM1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Gfm1,COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1; G translation elongation factor, mitochondrial; elongation factor G, mitochondrial; mitochondrial elongation factor G; mitochondrial elongation factor G1; G elongation factor, mitochondrial 1
Search name
Mouse Gfm1 ELISA KIT ,Mouse COXPD1 ELISA KIT ,Mouse EFG ELISA KIT ,Mouse EFG1 ELISA KIT ,Mouse EFGM ELISA KIT ,Mouse EGF1 ELISA KIT ,Mouse GFM ELISA KIT ,Mouse hEFG1 ELISA KIT ,Mouse G translation elongation factor mitochondrial ELISA KIT ,Mouse elongation factor G mitochondrial ELISA KIT ,Mouse mitochondrial elongation factor G ELISA KIT ,Mouse mitochondrial elongation factor G1 ELISA KIT ,Mouse G elongation factor mitochondrial 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Elongation factor G, mitochondrial, GFM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elongation factor G. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Elongation factor G and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Elongation factor G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Elongation factor G in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验多肽链延伸因子 polypeptide chain elonga-tion factor
蛋白质生物合成中参与多肽链延伸过程的蛋白质因子。从大肠杆菌等原核细胞可纯化获得三种延伸因子(elongation factor),即EFTu,EFTs和EFG,分子量大约分别为4.7万,3.6万和8.3万。EFTu与GTP结合成EFTu-GTP,然后再与氨酰tRNA结合,形成三复合体(ternary complex)氨酰-tRNA-EFTu-GTP。这种三复合体与核糖体的氨酰tRNA部位(A部位,aminoacyl site)结合,继而GTP被分解,EFTu以EFTu-
The fusion protein toxins that have been described are generally composed of the catalytic and transmembrane domains of a bacterial toxin (e.g., diphtheria toxin [DT] or Pseudomonas exotoxin A) to which a polypeptide hormone, growth factor
Craig, C.G., Tropepe, V., Morshead, C.M., Reynolds, B.A., Weiss, S., and van der Kooy, D. 1996. In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain. J
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