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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
FUN14 domain-containing protein 1, Fundc1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse FUN14 domain- containing protein 1, Fundc1 ELISA KIT
Product Name:Mouse FUN14 domain- containing protein 1, Fundc1 ELISA KIT
Packing:96T
Catalog No.:ELI-27558m
Gene Name:Mouse Fundc1
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Fundc1
Alternative Name:Mouse FUN14 domain-containing protein 1, Fundc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse FUN14 domain- containing protein 1, Fundc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Fundc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse FUN14 domain- containing protein 1, Fundc1 ELISA KIT has been pre-coated with an Mouse FUN14 domain-containing protein 1, Fundc1 antibody specific to Mouse Fundc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fundc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fundc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fundc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Introduction of human 14-3-3 proteins
----------------------------------------------------------------------------A binding sequence for the 14-3-3 protein within the cytoplasmic domain of the adhesion receptor,platelet glycoprotein Ib alphaDu X; Fox JE; Pei SDepartment of Vascular Biology,Scripps Research Institute,La Jolla,California
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
can be effectively homogenized by pipetting up and down or vortexing after addition of Cell lysis buffer. 3. Place the tube containing the homogenates on the bench at room temperature for 2 minutes. 4. Add 100 μL of Protein Precipitation Reagent
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