UDP-N-acetylglucosamine--dolic

Mouse UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DPAGT1,ALG7; CDG-Ij; CDG1J; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra; dolichyl-phosphate alpha-N-acetylglucosaminyltransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)

Search name

Mouse DPAGT1 ELISA KIT ,Mouse ALG7 ELISA KIT ,Mouse CDG-Ij ELISA KIT ,Mouse CDG1J ELISA KIT ,Mouse CMSTA2 ELISA KIT ,Mouse D11S366 ELISA KIT ,Mouse DGPT ELISA KIT ,Mouse DPAGT ELISA KIT ,Mouse DPAGT2 ELISA KIT ,Mouse G1PT ELISA KIT ,Mouse GPT ELISA KIT ,Mouse UAGT ELISA KIT ,Mouse UGAT ELISA KIT ,Mouse GlcNAc-1-P transferase ELISA KIT ,Mouse N-acetylglucosamine-1-phosphate transferase ELISA KIT ,Mouse UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Mouse UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Mouse dolichyl-phosphate ELISA KIT ,Mouse UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra ELISA KIT ,Mouse dolichyl-phosphate alpha-N-acetylglucosaminyltransferase ELISA KIT ,Mouse dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPAGT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DPAGT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DPAGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPAGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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