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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetyl
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DPAGT1,ALG7; CDG-Ij; CDG1J; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra; dolichyl-phosphate alpha-N-acetylglucosaminyltransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Search name
Mouse DPAGT1 ELISA KIT ,Mouse ALG7 ELISA KIT ,Mouse CDG-Ij ELISA KIT ,Mouse CDG1J ELISA KIT ,Mouse CMSTA2 ELISA KIT ,Mouse D11S366 ELISA KIT ,Mouse DGPT ELISA KIT ,Mouse DPAGT ELISA KIT ,Mouse DPAGT2 ELISA KIT ,Mouse G1PT ELISA KIT ,Mouse GPT ELISA KIT ,Mouse UAGT ELISA KIT ,Mouse UGAT ELISA KIT ,Mouse GlcNAc-1-P transferase ELISA KIT ,Mouse N-acetylglucosamine-1-phosphate transferase ELISA KIT ,Mouse UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Mouse UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Mouse dolichyl-phosphate ELISA KIT ,Mouse UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra ELISA KIT ,Mouse dolichyl-phosphate alpha-N-acetylglucosaminyltransferase ELISA KIT ,Mouse dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPAGT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DPAGT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DPAGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPAGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Study of the Biological Functions of Mucin Type Core 3 O-glycans
UDP-N -acetylglucosamine as substrate. We revealed that the core 3 O -glycan structure modulates prostate cancer formation and gastrointestinal cell differentiation through regulating the heterodimerization of α2β1 integrin [1, 2] and cell surface
尿苷二磷酸-N-Z酰葡糖胺 uridine dipho-sphate N-acetylglucosamine
简称 UDP-N-乙酰葡糖胺。系核苷酸糖的一种,由 N-乙酰萄糖胺 -1-磷酸和 UTP生物合成。对于细菌细胞的肽葡聚糖( peptidoglycan)、酯多糖、霉菌的聚乙酰氨基葡糖、动物的透明质酸、肝素、糖蛋白、糖脂的生物合成可作为 N-乙酰葡糖胺残基的供体。此外,许多细胞具有 UDP-N-乙酰葡糖胺 -4-差向异构酶( UDP-N-acetylglucosamine-4-epim-erase, EC5. 1. 3. 7)由于此酶的作用发生
Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II: Cell Wall Analysis
of this resistance mechanism. The rate of the cell-wall synthesis can be evaluated by measuring the rate of up-take of 14 C-N-acetylglucosamine into the cell, since more than 95% of 14 C-N-acetylglucosamine is known to be incorporated into the cell wall (1 ). Murein
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