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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Hematopoietic prostaglandin D synthase, Hpgds
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Hematopoietic prostaglandin D synthase, HPGDS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Hematopoietic prostaglandin D synthase,H-PGDS,GST, GST TAG,Prostaglandin-H2 D-isomerase,Glutathione-requiring prostaglandin D synthase,Glutathione-dependent PGD synthase,GST class-sigma,Glutathione S-transferase HpgdsH-PGDS; Ptgds2; GST class-sigma; glutathione S-transferase; glutathione-dependent PGD synthase; glutathione-dependent PGD synthetase; glutathione-requiring prostaglandin D synthase; prostaglandin D2 synthase 2, hematopoietic; prostaglandin-H2 D-isomerase; hematopoietic prostaglandin D synthase
Search name
Mouse Hematopoietic prostaglandin D synthase ELISA KIT ,Mouse H-PGDS ELISA KIT ,Mouse GST ELISA KIT ,Mouse GST TAG ELISA KIT ,Mouse Prostaglandin-H2 D-isomerase ELISA KIT ,Mouse Glutathione-requiring prostaglandin D synthase ELISA KIT ,Mouse Glutathione-dependent PGD synthase ELISA KIT ,Mouse GST class-sigma ELISA KIT ,Mouse Glutathione S-transferase HpgdsH-PGDS ELISA KIT ,Mouse Ptgds2 ELISA KIT ,Mouse GST class-sigma ELISA KIT ,Mouse glutathione S-transferase ELISA KIT ,Mouse glutathione-dependent PGD synthase ELISA KIT ,Mouse glutathione-dependent PGD synthetase ELISA KIT ,Mouse glutathione-requiring prostaglandin D synthase ELISA KIT ,Mouse prostaglandin D2 synthase 2 ELISA KIT ,Mouse hematopoietic ELISA KIT ,Mouse prostaglandin-H2 D-isomerase ELISA KIT ,Mouse hematopoietic prostaglandin D synthase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse hematopoietic prostaglandin d synthase,H-PGDS concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to H-PGDS, During the reaction, H-PGDS in the sample or standard competes with a fixed amount of biotin-labeled H-PGDS for sites on a pre-coated Monoclonal antibody specific to H-PGDS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H-PGDS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Support Protocol 3: Mouse Euthanasia and Tissue Dissection Support Protocol 4: Serum Insulin Quantification Using ELISA Support Protocol 5: Analysis of Human Cell Engraftment by Flow Cytometry
Models of Inflammation: Carrageenan Air Pouch
) ELISA kit(s) specific for inflammatory mediators (e.g., TNF‐α, IL‐1β, IL‐6, IL‐10, prostaglandins; Thermo Scientific) Isotonic phosphate‐buffered saline (PBS
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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