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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cyclin-O, Ccno
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cyclin- O, Ccno ELISA KIT
Product Name:Mouse Cyclin- O, Ccno ELISA KIT
Packing:96T
Catalog No.:ELI-25443m
Gene Name:Mouse Ccno
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ccno
Alternative Name:Mouse Cyclin-O, Ccno
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cyclin- O, Ccno ELISA KIT allows for the in vitro quantitative determination of Mouse Ccno concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cyclin- O, Ccno ELISA KIT has been pre-coated with an Mouse Cyclin-O, Ccno antibody specific to Mouse Ccno .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ccno and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ccno , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ccno in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验酶联免疫吸附试验是用酶标记的抗体(或SPA)检测未知抗原或抗体的方法。此法敏感性高,特异性强。常用的是ELISA方法,间接法,双抗体法和抗原法。本次试验介绍,酶联葡萄球菌蛋白A免疫分析法。 1、加抗原使之吸附于固相载体(如塑料反应板) 2、洗涤加待测血清 3、洗涤加酶标记SpA 4、洗涤加酶的底物。经酶的催化产生有色产物。 一、材料: 1、聚乙烯塑料反应板(PH9.6时可吸附
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 Ghrelin ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Ghrelin 单抗包被于酶标板上,标准品和样品中的 Ghrelin与单抗结合,加入生物素化的抗人
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