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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Death-associated protein kinase 1, Dapk1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Death- associated protein kinase 1, Dapk1 ELISA KIT
Product Name:Mouse Death- associated protein kinase 1, Dapk1 ELISA KIT
Packing:96T
Catalog No.:ELI-26386m
Gene Name:Mouse Dapk1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Dapk1
Alternative Name:Mouse Death-associated protein kinase 1, Dapk1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Death- associated protein kinase 1, Dapk1 ELISA KIT allows for the in vitro quantitative determination of Mouse Dapk1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Death- associated protein kinase 1, Dapk1 ELISA KIT has been pre-coated with an Mouse Death-associated protein kinase 1, Dapk1 antibody specific to Mouse Dapk1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dapk1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dapk1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dapk1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Assay for Isolation of Inhibitors of Her2-Kinase Expression
Her2 (ErbB2) protein is overexpressed in breast and other solid tumors, and its expression is associated with progressive disease. Current therapies directed toward Her2 either block dimerization of the receptor or inhibit tyrosine kinase
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
profile scanning approach for genome‐wide prediction of signaling pathways. Nat. Biotechnol. 19:348‐353. Yang, X., Hubbard, E.J., and Carlson, M. 1992. A protein kinase
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