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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Neuronal migration protein doublecortin, Dcx
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Neuronal migration protein doublecortin, Dcx ELISA KIT
Product Name:Mouse Neuronal migration protein doublecortin, Dcx ELISA KIT
Packing:96T
Catalog No.:ELI-26363m
Gene Name:Mouse Dcx
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Dcx
Alternative Name:Mouse Neuronal migration protein doublecortin, Dcx
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Neuronal migration protein doublecortin, Dcx ELISA KIT allows for the in vitro quantitative determination of Mouse Dcx concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Neuronal migration protein doublecortin, Dcx ELISA KIT has been pre-coated with an Mouse Neuronal migration protein doublecortin, Dcx antibody specific to Mouse Dcx .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dcx and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dcx , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dcx in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Immunocytochemical Analysis of Neuronal Differentiation
Doublecortin (Dcx), the early expressed marker Hu, and mature neuronal marker NeuN. In Chapters 25 and 27 of Part IV, we provide protocols for identifying newborn cells by using the mitotic label bromodeoxyuridine and for examining axonal projections by using
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
, such as doublecortin (DCX) or neuronal nuclei protein (NeuN). Curr. Protoc. Stem Cell Biol. 24:2D.14.1?2D.14.24. © 2013 by John Wiley & Sons, Inc. Keywords: EdU; BrdU; proliferation; subventricular zone; hippocampus
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