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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Spliceosome RNA helicase Ddx39b, Ddx39b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Spliceosome RNA helicase Ddx39b, Ddx39b ELISA KIT
Product Name:Mouse Spliceosome RNA helicase Ddx39b, Ddx39b ELISA KIT
Packing:96T
Catalog No.:ELI-26141m
Gene Name:Mouse Ddx39b
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ddx39b
Alternative Name:Mouse Spliceosome RNA helicase Ddx39b, Ddx39b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Spliceosome RNA helicase Ddx39b, Ddx39b ELISA KIT allows for the in vitro quantitative determination of Mouse Ddx39b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Spliceosome RNA helicase Ddx39b, Ddx39b ELISA KIT has been pre-coated with an Mouse Spliceosome RNA helicase Ddx39b, Ddx39b antibody specific to Mouse Ddx39b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ddx39b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ddx39b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ddx39b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
师从施一公,发表 6 篇Science/3篇 Cell,她被誉为「世界最具潜力女科学家」| 论文盘点
-B 和 B 复合体的冷冻电镜结构(图片来源:Science) 9. Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2 (Science,26 Nov 2020) 通过阻滞方法获得了高分辨率的原位酿酒酵母剪接体(Bact complex)的电镜结构,解析了 ATP 酶/解旋酶 Prp2 及其共激活剂 Spp2 重塑剪接体的机制,是国际上第一篇剪接体重塑机制的结构研究
万蕊雪/施一公等再发Science开辟新「地图」,这几个领域将直接受益
.org/cgi/doi/10.1126/science.abg0879[9]Wan R, Bai R, Zhan X, Shi Y. How Is Precursor Messenger RNA Spliced by the Spliceosome? Annu Rev Biochem. 2020 Jun 20;89:333-358. doi: 10.1146/annurev-biochem-013118-111024. Epub 2019 Dec 9. PMID: 31815536.题图来源:网络
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