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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Collagen alpha-4(VI) chain, Col6a4
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Collagen alpha- 4(VI) chain, Col6a4 ELISA KIT
Product Name:Mouse Collagen alpha- 4(VI) chain, Col6a4 ELISA KIT
Packing:96T
Catalog No.:ELI-25888m
Gene Name:Mouse Col6a4
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Col6a4
Alternative Name:Mouse Collagen alpha-4(VI) chain, Col6a4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Collagen alpha- 4(VI) chain, Col6a4 ELISA KIT allows for the in vitro quantitative determination of Mouse Col6a4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Collagen alpha- 4(VI) chain, Col6a4 ELISA KIT has been pre-coated with an Mouse Collagen alpha-4(VI) chain, Col6a4 antibody specific to Mouse Col6a4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Col6a4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Col6a4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Col6a4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
has also been found in rats in which EAE was induced by a peptide of myelin basic protein (5 ). Similarly, a restriction in the TCR usage was found also in CII-induced arthritis in mice (6 ,7 ), and in the TCR alpha chain usage in non-obese diabetic (NOD) mice (8 ).
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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