Polyribonucleotide 5'-hydroxyl
-kinase Clp1, Clp1 ELISA K/ Mouse Polyribonucleotide 5'-hydroxyl-kinase Clp1, Clp1 ELISA K- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-25819m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Polyribonucleotide 5'-hydroxyl-kinase Clp1, Clp1 ELISA K
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HEAB,pre-mRNA cleavage complex II protein Clp1; polyribonucleotide 5-hydroxyl-kinase Clp1; polynucleotide kinase Clp1; polyadenylation factor Clp1; CLP1, cleavage and polyadenylation factor I subunit, homolog; hClp1; ATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; cleavage and polyadenylation factor I subunit 1
Search name
Mouse HEAB ELISA KIT ,Mouse pre-mRNA cleavage complex II protein Clp1 ELISA KIT ,Mouse polyribonucleotide 5-hydroxyl-kinase Clp1 ELISA KIT ,Mouse polynucleotide kinase Clp1 ELISA KIT ,Mouse polyadenylation factor Clp1 ELISA KIT ,Mouse CLP1 ELISA KIT ,Mouse cleavage and polyadenylation factor I subunit homolog ELISA KIT ,Mouse hClp1 ELISA KIT ,Mouse ATP/GTP-binding protein ELISA KIT ,Mouse homolog of yeast CFIA subunit Clp1p ELISA KIT ,Mouse cleavage and polyadenylation factor I subunit 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CLP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CLP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
ELISPOT (Enzyme-linked ImmunoSPOT)
(Millipore, Cat. No. MAIPS4510) AEC substrate (Sigma, Cat. No. A-5754) Distilled water Wash Buffer: 1 x PBS, 0.05% Tween-20 (or eBioscience ELISA Wash Buffer Powder, cat 00-0400) Instruments Pipettes and pipettors
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