Mouse Uncharacterized protein C17orf80 homolog, D11Wsu47e ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
D11Wsu47e,uncharacterized protein C17orf80 homolog; DNA segment, Chr 11, Wayne State University 47, expressed
Search name
Mouse D11Wsu47e ELISA KIT ,Mouse uncharacterized protein C17orf80 homolog ELISA KIT ,Mouse DNA segment Chr 11 ELISA KIT ,Mouse Wayne State University 47 expressed ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Uncharacterized protein C17orf80 homolog, D11Wsu47e concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to D11Wsu47e. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D11Wsu47e and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D11Wsu47e, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D11Wsu47e in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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