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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
B-cell differentiation antigen CD72, Cd72
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse B- cell differentiation antigen CD72, Cd72 ELISA KIT
Product Name:Mouse B- cell differentiation antigen CD72, Cd72 ELISA KIT
Packing:96T
Catalog No.:ELI-25432m
Gene Name:Mouse Cd72
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cd72
Alternative Name:Mouse B-cell differentiation antigen CD72, Cd72
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse B- cell differentiation antigen CD72, Cd72 ELISA KIT allows for the in vitro quantitative determination of Mouse Cd72 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse B- cell differentiation antigen CD72, Cd72 ELISA KIT has been pre-coated with an Mouse B-cell differentiation antigen CD72, Cd72 antibody specific to Mouse Cd72 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cd72 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cd72 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cd72 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验B-Cell Development and Pre-B-1 Cell Plasticity In Vitro
have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test
Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow
. Different protocols have been developed to differentiate DC from total mouse bone marrow in vitro. Here, we describe the isolation of a specific DC progenitor population, referred to as preimmunocytes, and document protocols for their differentiation
In Vitro Effects of Adjuvants on B Cells
Stimulation of B cells not only through the B cell antigen receptor (BCR) but also through Toll-like receptors (TLRs) can drive activation, proliferation, and differentiation of B cells to result in antigen-specific antibody secretion
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