Centrosomal protein of 89 kDa,

Mouse Centrosomal protein of 89 kDa, CEP89 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CEP89,CCDC123; CEP123; centrosomal protein 123; centrosomal protein of 89 kDa; coiled-coil domain containing 123; coiled-coil domain-containing protein 123, mitochondrial; centrosomal protein 89kDa

Search name

Mouse CEP89 ELISA KIT ,Mouse CCDC123 ELISA KIT ,Mouse CEP123 ELISA KIT ,Mouse centrosomal protein 123 ELISA KIT ,Mouse centrosomal protein of 89 kDa ELISA KIT ,Mouse coiled-coil domain containing 123 ELISA KIT ,Mouse coiled-coil domain-containing protein 123 ELISA KIT ,Mouse  mitochondrial ELISA KIT ,Mouse centrosomal protein 89kDa ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Centrosomal protein of 89 kDa, CEP89 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CEP89. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CEP89 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CEP89, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CEP89 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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