Actin-like protein 7A, Actl7a
ELISA KIT/ Mouse Actin-like protein 7A, Actl7a ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-24803m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Actin-like protein 7A, Actl7a
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Actin- like protein 7A, Actl7a ELISA KIT
Product Name:Mouse Actin- like protein 7A, Actl7a ELISA KIT
Packing:96T
Catalog No.:ELI-24803m
Gene Name:Mouse Actl7a
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Actl7a
Alternative Name:Mouse Actin-like protein 7A, Actl7a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Actin- like protein 7A, Actl7a ELISA KIT allows for the in vitro quantitative determination of Mouse Actl7a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Actin- like protein 7A, Actl7a ELISA KIT has been pre-coated with an Mouse Actin-like protein 7A, Actl7a antibody specific to Mouse Actl7a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Actl7a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Actl7a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Actl7a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolate It All：siRNA • miRNA • Total RNA • Native Protein
Figure 5. Isolation of Total RNA and Protein from Different Tissues. Total RNA and protein were isolated from 4 different mouse tissues (~50 mg) with the mir Vana™ miRNA Isolation Kit (RNA only) or the mir Vana™ PARIS™ Kit. (A) Small RNA
Use of Internaland External Standardsor Reference RNAs for A
Tissues Using a Ribonuclease Protection Assay. High specific activity antisense RNA probes (1.15 x 109 cpm/µg) to GAPDH, ß-actin and cyclophilin were synthesized from Ambion's pTRI-GAPDH, pTRI-beta-actin, and pTRI-cyclophilin mouse DNA templates
Use of Internal and External Standards or Reference RNAs for
a Ribonuclease Protection Assay. High specific activity antisense RNA probes (1.15 x 10 9 cpm/µg) to GAPDH, ß-actin and cyclophilin were synthesized from Ambion's pTRI-GAPDH, pTRI-beta-actin, and pTRI-cyclophilin mouse DNA
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