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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Adenosine kinase, Adk
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Adenosine kinase, Adk ELISA KIT
Product Name:Mouse Adenosine kinase, Adk ELISA KIT
Packing:96T
Catalog No.:ELI-24595m
Gene Name:Mouse Adk
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Adk
Alternative Name:Mouse Adenosine kinase, Adk
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Adenosine kinase, Adk ELISA KIT allows for the in vitro quantitative determination of Mouse Adk concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Adenosine kinase, Adk ELISA KIT has been pre-coated with an Mouse Adenosine kinase, Adk antibody specific to Mouse Adk .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Adk and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Adk , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Adk in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
Adenosine is an important modulator of metabolic activity with powerful tissue- and cell-protective functions. Adenosine kinase (ADK), the major adenosine-regulating enzyme, is critical to adapt its intra- and extra-cellular levels
Assays for the Interferon-Induced Enzyme 2,5 Oligoadenylate Synthetases
Inhibition of protein synthesis by interferon treatment is mediated by two major pathways: the 2′–5′-linked oligoadenylates [2–5 (A)] synthetase-RNase L pathway and the double-stranded ribonucleic acid-dependent protein kinase-mediated
----------------------- Screening for Protein-Protein Interactions. Protein G was from Pierce (Rockford, IL) and BODIPY-FL-Goat-anti-Mouse IgG was from Molecular Probes (Eugene, OR). IKBalpha and p50 were kindly provided by T. Maniatis (Harvard University, Cambridge, MA
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