- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-24299m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Serine
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Serine/threonine- protein kinase A- Raf, Araf ELISA KIT
Product Name:Mouse Serine/threonine- protein kinase A- Raf, Araf ELISA KIT
Packing:96T
Catalog No.:ELI-24299m
Gene Name:Mouse Araf
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Araf
Alternative Name:Mouse Serine/threonine-protein kinase A-Raf, Araf
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine/threonine- protein kinase A- Raf, Araf ELISA KIT allows for the in vitro quantitative determination of Mouse Araf concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Serine/threonine- protein kinase A- Raf, Araf ELISA KIT has been pre-coated with an Mouse Serine/threonine-protein kinase A-Raf, Araf antibody specific to Mouse Araf .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Araf and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Araf , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Araf in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验The Raf-1 serine/threonine protein kinase is a central component of the Ras/Raf/MEK/MAP kinase cascade, a highly conserved signaling pathway responsible for the transmission of signals emanating from cell surface growth factor, cytokine
Raf-1 Protein Kinase Activity in T Cells from Aged Mice
Most of our models of signal transduction through the T-cell receptor (TCR) involve components and pathways first described in T-cell clones and T-cell lymphomas such as the Jurkat cell line (1 ). These studies, while providing valuable
The progress made in understanding the molecular basis of mammalian cell transformation has led to the unifying concept of growth regulation and its disorders in cancer cells. Today it is well recognized that many products of “cancer genes
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