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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Nuclear receptor-binding protein 2, Nrbp2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nuclear receptor- binding protein 2, Nrbp2 ELISA KIT
Product Name:Mouse Nuclear receptor- binding protein 2, Nrbp2 ELISA KIT
Packing:96T
Catalog No.:ELI-23713m
Gene Name:Mouse Nrbp2
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Nrbp2
Alternative Name:Mouse Nuclear receptor-binding protein 2, Nrbp2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nuclear receptor- binding protein 2, Nrbp2 ELISA KIT allows for the in vitro quantitative determination of Mouse Nrbp2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nuclear receptor- binding protein 2, Nrbp2 ELISA KIT has been pre-coated with an Mouse Nuclear receptor-binding protein 2, Nrbp2 antibody specific to Mouse Nrbp2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nrbp2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nrbp2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nrbp2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Nuclear Receptor Acetylation
Acetylation is an essential post-translational modification in which an acetyl group is covalently conjugated to a protein substrate. Histone acetylation was first proposed nearly half a century ago by Dr. Vincent Allfrey. Subsequent studies
Large Scale Production of Nuclear Receptor Ligand-Binding Domains
The nuclear receptor superfamily is composed of over 150 inducible transcription factors, most of which (1 -2 ) do not have well characterized ligands to date. Nuclear receptors regulate promoters through specific protein-protein
Thioredoxin and Redox Regulation of the Nuclear Receptor
Oxidative stress evokes various cellular responses including alteration of gene expression to preserve cellular homeostasis (1 ,2 ). Thioredoxin (TRX) is a small ubiquitous protein with protein thiol-reducing activity and has been shown
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