- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-23494m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Nuclear valosin-containing protein-like, Nvl
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Nuclear valosin- containing protein- like, Nvl ELISA KIT
Product Name:Mouse Nuclear valosin- containing protein- like, Nvl ELISA KIT
Packing:96T
Catalog No.:ELI-23494m
Gene Name:Mouse Nvl
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Nvl
Alternative Name:Mouse Nuclear valosin-containing protein-like, Nvl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nuclear valosin- containing protein- like, Nvl ELISA KIT allows for the in vitro quantitative determination of Mouse Nvl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nuclear valosin- containing protein- like, Nvl ELISA KIT has been pre-coated with an Mouse Nuclear valosin-containing protein-like, Nvl antibody specific to Mouse Nvl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nvl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nvl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nvl in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Elite ABC kit) in 1 mL of PBS containing 0.1% BSA for 10 min at room temperature. ix. After incubation with secondary antibodies, rinse slides twice in PBS containing 0.1% BSA (10 min each). Remove excess liquid
) Nuclear lysis buffer Oligonucleotide linkers (optional; see Step 18) PBS (phosphate-buffered saline [pH 7.4]) PCR product purification kit (e.g., QiaQuick PCR purification kit, QIAGEN) PCR reagents (e.g., Taq polymerase
RNAse A Treatment of Mouse Cells
of the quality of the used RNAse A. In our lab, we used RNAse A treatment of mouse cells (see comment 1 ) to demonstrate that the enrichment in HP1 (Heterochromatin Protein 1) proteins at pericentric heterochromatin depends on the presence of an RNA component
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