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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
ADP-ribose pyrophosphatase mitochondrial, Nudt9
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse ADP- ribose pyrophosphatase mitochondrial, NUDT9 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NUDT9,NUDT10,PSEC0099,Nucleoside diphosphate-linked moiety X motif 9,Nudix motif 9;Adenosine diphosphoribose pyrophosphatase,ADPR-PPase;ADP-ribose diphosphatase;ADP-ribose phosphohydrolase,ADP-ribose pyrosphosphatase NUDT9; ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ADP-ribose pyrophosphatase mitochondrial; nudix (nucleoside diphosphate linked moiety X)-type motif 9
Search name
Mouse NUDT9 ELISA KIT ,Mouse NUDT10 ELISA KIT ,Mouse PSEC0099 ELISA KIT ,Mouse Nucleoside diphosphate-linked moiety X motif 9 ELISA KIT ,Mouse Nudix motif 9 ELISA KIT ,Mouse Adenosine diphosphoribose pyrophosphatase ELISA KIT ,Mouse ADPR-PPase ELISA KIT ,Mouse ADP-ribose diphosphatase ELISA KIT ,Mouse ADP-ribose phosphohydrolase ELISA KIT ,Mouse ADP-ribose pyrosphosphatase NUDT9 ELISA KIT ,Mouse ADP-ribose diphosphatase ELISA KIT ,Mouse ADP-ribose phosphohydrolase ELISA KIT ,Mouse ADP-ribose pyrophosphatase mitochondrial ELISA KIT ,Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 9 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse ADP- ribose pyrophosphatase, mitochondrial, NUDT9 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP- ribose pyrophosphatase, mitochondrial, NUDT9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse ADP- ribose pyrophosphatase, mitochondrial, NUDT9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse ADP- ribose pyrophosphatase, mitochondrial, NUDT9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse ADP- ribose pyrophosphatase, mitochondrial, NUDT9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue
. We also include a method of measuring ADP-ATP exchange rate as a function of mitochondrial membrane potential (ΔΨm) in small amounts of isolated mitochondria, adapted to a plate reader format.
In Vivo and In Vitro Determination of Cell Death Markers in Neurons
:621–632, 2010 and Galluzzi et al., Apoptosis 12:803–813, 2007). Thus, any perturbation of outer or inner mitochondrial membranes can lead to disruptions in the normal fluxes of key ions and metabolic proteins (i.e., ADP/ATP exchange), leading
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
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