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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
MLX-interacting protein, Mlxip
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse MLX- interacting protein, Mlxip ELISA KIT
Product Name:Mouse MLX- interacting protein, Mlxip ELISA KIT
Packing:96T
Catalog No.:ELI-23481m
Gene Name:Mouse Mlxip
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Mlxip
Alternative Name:Mouse MLX-interacting protein, Mlxip
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse MLX- interacting protein, Mlxip ELISA KIT allows for the in vitro quantitative determination of Mouse Mlxip concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse MLX- interacting protein, Mlxip ELISA KIT has been pre-coated with an Mouse MLX-interacting protein, Mlxip antibody specific to Mouse Mlxip .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mlxip and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mlxip , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mlxip in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phage‐Based Expression Cloning to Identify Interacting Proteins
expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
pentagon, bait protein; open rectangle, noninteracting library protein; shaded box, activator protein (acid blob in Fig. ); open chevron, interacting library protein. View Image
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
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