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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Nuclear factor NF-kappa-B p100 subunit, Nfkb2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nuclear factor NF- kappa- B p100 subunit, Nfkb2 ELISA KIT
Product Name:Mouse Nuclear factor NF- kappa- B p100 subunit, Nfkb2 ELISA KIT
Packing:96T
Catalog No.:ELI-23173m
Gene Name:Nfkb2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse Nuclear factor NF- kappa- B p100 subunit
Alternative Name:Nfkb2,Mouse Nuclear factor NF- kappa- B p100 subunit
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nuclear factor NF- kappa- B p100 subunit, Nfkb2 ELISA KIT allows for the in vitro quantitative determination ofMouse Nuclear factor NF- kappa- B p100 subunit concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nuclear factor NF- kappa- B p100 subunit, Nfkb2 ELISA KIT has been pre-coated with an Nfkb2 antibody specific to Mouse Nuclear factor NF- kappa- B p100 subunit .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nuclear factor NF- kappa- B p100 subunit and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nuclear factor NF- kappa- B p100 subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nuclear factor NF- kappa- B p100 subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验The activation of NF-κB:The activation of NF-κB. Cell signaling pathways induced by growth factors, chemotherapy, or radiotherapy result in the phosphorylation and proteasomal degradation of IκB. The transcription factor NF-κB
, several others have been identified in this family of transcription factors: P49, c- rel , p100, rel B, dorsal , Bcl-3, and p105 ( 1 ). The NF-kheterodimer is associated in the cytoplasm with the IkB subunit, which sequesters this factor in an inactive state ( 7 ). On activation
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
), nuclear gene encoding mitochondrial protein, mRNA 2307 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog 1 (ARAF1), mRNA 846 NM_002967 Homo sapiens scaffold attachment factor B (SAFB ), mRNA 545 NM_001183 Homo sapiens ATPase
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