Lon protease homolog, mitochon
drial, Lonp1 ELISA KIT/ Mouse Lon protease homolog, mitochondrial, Lonp1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22880m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Lon protease homolog, mitochondrial, Lonp1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Lon protease homolog mitochondrial,LONP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LONP1,LON; LONP; LonHS; PIM1; PRSS15; hLON; hLON ATP-dependent protease; lon protease homolog, mitochondrial; mitochondrial ATP-dependent protease Lon; mitochondrial lon protease-like protein; serine protease 15; lon peptidase 1, mitochondrial
Search name
Mouse LONP1 ELISA KIT ,Mouse LON ELISA KIT ,Mouse LONP ELISA KIT ,Mouse LonHS ELISA KIT ,Mouse PIM1 ELISA KIT ,Mouse PRSS15 ELISA KIT ,Mouse hLON ELISA KIT ,Mouse hLON ATP-dependent protease ELISA KIT ,Mouse lon protease homolog ELISA KIT ,Mouse mitochondrial ELISA KIT ,Mouse mitochondrial ATP-dependent protease Lon ELISA KIT ,Mouse mitochondrial lon protease-like protein ELISA KIT ,Mouse serine protease 15 ELISA KIT ,Mouse lon peptidase 1 mitochondrial ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Lon protease homolog mitochondrial,LONP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LONP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LONP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LONP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LONP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
), nuclear gene encoding mitochondrial protein, mRNA 2307 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog 1 (ARAF1), mRNA 846 NM_002967 Homo sapiens scaffold attachment factor B (SAFB ), mRNA 545 NM_001183 Homo sapiens ATPase
细胞中，它在线粒体中形成聚集体，发出强烈的红色荧光。凋亡细胞中，因线粒体穿膜电位的改变，它以单体形式存在于细胞液中，发出绿色荧光。用荧光显微镜或流式细胞仪可清楚地分辨这两种不同的荧光信号。CLONTECH公司的ApoAlert Mitochondrial Membrane Sensor Kit就采用这种原理来检测线粒体膜电位的变化。但是，这种方法不能区分细胞凋亡或其他原因导致的线粒体膜电位的变化。 2. 晚期检测 细胞凋亡晚期中，核酸内切酶(某些Caspase的底物)在核小体之间剪切
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