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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Vitamin D-binding protein, Gc含量
- 适应物种:
小鼠
- 标记物:
Vitamin D-binding protein, Gc
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Vitamin D-binding protein, Gc ELISA试剂盒
Product Name: Mouse Vitamin D-binding protein, Gc ELISA试剂盒
Product Name: Mouse Vitamin D-binding protein, Gc ELISA试剂盒
Gene Name: Mouse Vitamin D-binding protein, Gc
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Vitamin D-binding protein, Gc
Alternative Name: Mouse Vitamin D-binding protein, Gc
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Vitamin D-binding protein, Gc ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Vitamin D-binding protein, Gc concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Vitamin D-binding protein, Gc ELISA试剂盒 has been pre-coated with an Mouse Vitamin D-binding protein, Gc antibody specific to Mouse Vitamin D-binding protein, Gc .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Vitamin D-binding protein, Gc and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Vitamin D-binding protein, Gc , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Vitamin D-binding protein, Gc in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN Indirect Method Materials 1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802), prepare 2 mg/ml stock in PBS solution A, clarify 25,000 rpm for 20 min in a 42
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
. A physicochemical study f protein G, a molecule with unique immunoglobulin G-binding properties. J. Biol. Chem. 261:10240-10247. Harlow, E. and Lane, D. 1988. Antibodies. A laboratory manual. Cold Spring Harbor Laboratory. Kronvall, G., Seal, U. S
Steroid hormone receptors activate and repress gene transcription through an integrated series of protein-protein interactions. One principle contact is the interaction of the nuclear receptors with one another to form both homodimeric
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