Methyl-CpG-binding domain prot
ein 2, Mbd2 ELISA KIT/ Mouse Methyl-CpG-binding domain protein 2, Mbd2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22761m
- 2025年07月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Methyl-CpG-binding domain protein 2, Mbd2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Methyl- CpG- binding domain protein 2, Mbd2 ELISA KIT
Product Name:Mouse Methyl- CpG- binding domain protein 2, Mbd2 ELISA KIT
Packing:96T
Catalog No.:ELI-22761m
Gene Name:Mouse Mbd2
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Mbd2
Alternative Name:Mouse Methyl-CpG-binding domain protein 2, Mbd2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Methyl- CpG- binding domain protein 2, Mbd2 ELISA KIT allows for the in vitro quantitative determination of Mouse Mbd2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Methyl- CpG- binding domain protein 2, Mbd2 ELISA KIT has been pre-coated with an Mouse Methyl-CpG-binding domain protein 2, Mbd2 antibody specific to Mouse Mbd2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mbd2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mbd2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mbd2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
of methyl′CpG binding proteins was described 7 . The five members of this family share a similar methyl′CpG binding domain. One of these proteins, MeCP2, is known to be necessary for embryonic development, although the effects on imprinted genes
清楚，因此其需要后续进一步分析确定，此外，RLGS图谱不能完全确认所缺失的片段是由于甲基化所致还是由于DNA本身缺失所致[5]且结果分析复杂，不易解释。 2.3.2 MBD（Methyl-CpG binding domain column chromatography，甲基化结合区）柱层析法 根据MBD蛋白家族和MeCP2的特性，Masahiko Shiraishi等2004年[50]提出了一种新的方法MBD柱层析法，用于筛选和发现基因组中甲基化的情况。并且比较了MBD和重亚硫酸盐基因组测序法，得出：用MBD
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