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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Leukocyte-specific transcript 1 protein, Lst1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Leukocyte- specific transcript 1 protein, Lst1 ELISA KIT
Product Name:Mouse Leukocyte- specific transcript 1 protein, Lst1 ELISA KIT
Packing:96T
Catalog No.:ELI-22746m
Gene Name:Mouse Lst1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Lst1
Alternative Name:Mouse Leukocyte-specific transcript 1 protein, Lst1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Leukocyte- specific transcript 1 protein, Lst1 ELISA KIT allows for the in vitro quantitative determination of Mouse Lst1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Leukocyte- specific transcript 1 protein, Lst1 ELISA KIT has been pre-coated with an Mouse Leukocyte-specific transcript 1 protein, Lst1 antibody specific to Mouse Lst1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lst1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lst1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lst1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
sequences will be specific for the primary transcript (see Robert Singer's web site for more details on use of oligos as probes: http://singerlab.aecom.yu.edu/). For the detection of Xist RNA coating of the X chromosome in cis, or primary transcripts of X
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