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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Nuclease-sensitive element-binding protein 1, Ybx1 ELISA
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nuclease- sensitive element- binding protein 1, YBX1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
YBX1,YB 1,BP-8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1; CBF-A; CCAAT-binding transcription factor I subunit A; DNA-binding protein B; EFI-A; Y-box transcription factor; Y-box-binding protein 1; enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; nuclease sensitive element binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1
Search name
Mouse YBX1 ELISA KIT ,Mouse YB 1 ELISA KIT ,Mouse BP-8 ELISA KIT ,Mouse CSDA2 ELISA KIT ,Mouse CSDB ELISA KIT ,Mouse DBPB ELISA KIT ,Mouse MDR-NF1 ELISA KIT ,Mouse NSEP-1 ELISA KIT ,Mouse NSEP1 ELISA KIT ,Mouse YB-1 ELISA KIT ,Mouse YB1 ELISA KIT ,Mouse CBF-A ELISA KIT ,Mouse CCAAT-binding transcription factor I subunit A ELISA KIT ,Mouse DNA-binding protein B ELISA KIT ,Mouse EFI-A ELISA KIT ,Mouse Y-box transcription factor ELISA KIT ,Mouse Y-box-binding protein 1 ELISA KIT ,Mouse enhancer factor I subunit A ELISA KIT ,Mouse major histocompatibility complex ELISA KIT ,Mouse class II ELISA KIT ,Mouse Y box-binding protein I ELISA KIT ,Mouse nuclease sensitive element binding protein 1 ELISA KIT ,Mouse nuclease-sensitive element-binding protein 1 ELISA KIT ,Mouse Y box binding protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Nuclease- sensitive element- binding protein 1, YBX1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclease- sensitive element- binding protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Nuclease- sensitive element- binding protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nuclease- sensitive element- binding protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Nuclease- sensitive element- binding protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验Probing Chromatin Structure with Nuclease Sensitivity Assays
organization of chromatin characterized by nuclease hypersensitivity is present on only one of the two parental chromosome (3 ). For example, the differentially methylated 5′-portion of the human SNRPN gene-a sequence element that controls imprinting
applicability, each of these approaches provides only partial information about DNA-protein complexes. DNase I footprinting identifies the extent and location of the binding site within the DNA but does not yield information about the protein(s) involved
). Despite their broad applicability, these techniques provide only partial information about the DNA-protein system under investigation. The first three techniques identify either the site(s) of transcription factor binding within the DNA (size and location
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