Homeobox protein NANOG, Nanog
ELISA KIT/ Mouse Homeobox protein NANOG, Nanog ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22327m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Homeobox protein NANOG, Nanog
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Homeobox protein NANOG, Nanog ELISA KIT
Product Name:Mouse Homeobox protein NANOG, Nanog ELISA KIT
Packing:96T
Catalog No.:ELI-22327m
Gene Name:Mouse Nanog
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Nanog
Alternative Name:Mouse Homeobox protein NANOG, Nanog
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Homeobox protein NANOG, Nanog ELISA KIT allows for the in vitro quantitative determination of Mouse Nanog concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Homeobox protein NANOG, Nanog ELISA KIT has been pre-coated with an Mouse Homeobox protein NANOG, Nanog antibody specific to Mouse Nanog .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nanog and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nanog , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nanog in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Array‐Based High‐Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
of Feeder‐Free Mouse ESCs with an shRNA Lentivirus Library Basic Protocol 2: Select the Candidate Genes by alamarBlue Assay and Alkaline Phosphatase Assay Basic Protocol 3: Select the Candidate Genes Based
Intracellular Immunofluorescent Staining for Flow Cytometry
eBioGrB Mouse Langerin (CD207) eBioRMUL.2 Human Nanog hNanog.1 Human PCNA (Proliferating Cell Nuclear Antigen) PC10 Mouse Perforin eBioOMAK-D
Active Motif ChIP 技术专家经验分享：如何才能做好 ChIP 实验？
是有区别的。因为 ChIP 相对于 IP 需要甲醛交联的过程，甲醛会在 DNA 和蛋白质，以及蛋白和蛋白的氨基/亚氨基之间形成共价键，所以 ChIP 级的抗体还要识别甲醛交联后的目的蛋白。这跟免疫荧光的抗体有点类似。ChIP 级的抗体跟目的蛋白有足够强的相互作用，能够耐受高盐，低盐及多种去垢剂（比如 0.1% SDS，1% Triton X-100 或 NP-40）的清洗。所以选择抗体时，最好选择文献报道可以用于 ChIP 实验的抗体，或抗体公司提供 ChIP-qPCR 或 ChIP-seq 数据
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