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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Palmitoyl-protein thioesterase 1, PPT1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Palmitoyl-protein thioesterase 1, PPT1 ELISA Kit
Product Name:Mouse Palmitoyl-protein thioesterase 1, PPT1 ELISA Kit
Packing:96T
Catalog No.:ELI-21664m
Gene Name:PPT1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse Palmitoyl-protein thioesterase 1
Alternative Name:PPT1,Mouse Palmitoyl-protein thioesterase 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Palmitoyl-protein thioesterase 1, PPT1 ELISA Kit allows for the in vitro quantitative determination ofMouse Palmitoyl-protein thioesterase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Palmitoyl-protein thioesterase 1, PPT1 ELISA Kit has been pre-coated with an PPT1 antibody specific to Mouse Palmitoyl-protein thioesterase 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Palmitoyl-protein thioesterase 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Palmitoyl-protein thioesterase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Palmitoyl-protein thioesterase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
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