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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
PHD finger protein 23, Phf23
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse PHD finger protein 23, Phf23 ELISA KIT
Product Name:Mouse PHD finger protein 23, Phf23 ELISA KIT
Packing:96T
Catalog No.:ELI-21546m
Gene Name:Mouse Phf23
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Phf23
Alternative Name:Mouse PHD finger protein 23, Phf23
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse PHD finger protein 23, Phf23 ELISA KIT allows for the in vitro quantitative determination of Mouse Phf23 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse PHD finger protein 23, Phf23 ELISA KIT has been pre-coated with an Mouse PHD finger protein 23, Phf23 antibody specific to Mouse Phf23 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Phf23 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Phf23 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Phf23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
IF:29!华西刘玉、陈崇伉俪报道染色体缺失促进肿瘤发生的新机制,除了P53还有它
p deletion-driven tumorigenesis 的研究性论文,该研究利用多种功能遗传策略,鉴定出一个新的 17p 肿瘤抑制基因 —— PHD finger protein 23 (PHF23),研究发现,PHF23 的丢失会损害 B 细胞分化并促进未成熟的 B 淋巴细胞恶性肿瘤的发生。机制层面上,他们发现 PHF23 可通过 N - 末端直接与 SIN3-HDAC 复合物结合,并抑制其在 H3K27ac 上的去乙酰化活性。 图片来源:CANCER DISCOVERY 研究内容 PHF23 为单倍
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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