Poly [ADP-ribose] polymerase 1
6, Parp16 ELISA KIT/ Mouse Poly [ADP-ribose] polymerase 16, Parp16 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22007m
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Poly [ADP-ribose] polymerase 16, Parp16
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Poly [ADP- ribose] polymerase 16, Parp16 ELISA KIT
Product Name:Mouse Poly [ADP- ribose] polymerase 16, Parp16 ELISA KIT
Packing:96T
Catalog No.:ELI-22007m
Gene Name:Mouse Parp16
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Parp16
Alternative Name:Mouse Poly [ADP-ribose] polymerase 16, Parp16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Poly [ADP- ribose] polymerase 16, Parp16 ELISA KIT allows for the in vitro quantitative determination of Mouse Parp16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Poly [ADP- ribose] polymerase 16, Parp16 ELISA KIT has been pre-coated with an Mouse Poly [ADP-ribose] polymerase 16, Parp16 antibody specific to Mouse Parp16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Parp16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Parp16 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Parp16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Activity Assays for Poly-ADP Ribose Polymerase
Poly(ADP-ribose) polymerase (PARP-1) is a nuclear enzyme that has traditionally been thought to require discontinuous or “damaged” DNA (dcDNA) as a coenzyme, a preconception that has limited research mainly to its role in cell pathology, i.e
Purification of Recombinant Poly(ADP-Ribose) Polymerases
The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli ) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude
Assay for Protein Modification by Poly-ADP-Ribose In Vitro
The enzymatic function of poly(adenosine diphosphate (ADP)-ribose) polymerase (PARP) is central to many of its function as a component of DNA repair machinery, modulator of gene transcription, and cell differentiation. While the auto
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