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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
RNA binding protein fox-1 homolog 3, Rbfox3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse RNA binding protein fox- 1 homolog 3, Rbfox3 ELISA KIT
Product Name:Mouse RNA binding protein fox- 1 homolog 3, Rbfox3 ELISA KIT
Packing:96T
Catalog No.:ELI-21931m
Gene Name:Rbfox3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse RNA binding protein fox- 1 homolog 3
Alternative Name:Rbfox3,Mouse RNA binding protein fox- 1 homolog 3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RNA binding protein fox- 1 homolog 3, Rbfox3 ELISA KIT allows for the in vitro quantitative determination ofMouse RNA binding protein fox- 1 homolog 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RNA binding protein fox- 1 homolog 3, Rbfox3 ELISA KIT has been pre-coated with an Rbfox3 antibody specific to Mouse RNA binding protein fox- 1 homolog 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse RNA binding protein fox- 1 homolog 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse RNA binding protein fox- 1 homolog 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse RNA binding protein fox- 1 homolog 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolation of RNA from Difficult Tissues
ToTALLY RNA™ Kit). Note that many of these techniques can be used with other RNA isolation protocols as well. Heart and Skeletal Muscle: Fibrous Tissues For fibrous tissues such as rat and mouse heart and skeletal muscle, the most difficult step
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
Isolate It All:siRNA • miRNA • Total RNA • Native Protein
Figure 5. Isolation of Total RNA and Protein from Different Tissues. Total RNA and protein were isolated from 4 different mouse tissues (~50 mg) with the mir Vana™ miRNA Isolation Kit (RNA only) or the mir Vana™ PARIS™ Kit. (A) Small RNA
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