- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-06945m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Protein jagged-1, Jag1含量
- 适应物种：
小鼠
- 标记物：
Protein jagged-1, Jag1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein jagged- 1, JAG1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
JAGGED1,AGS; AHD; AWS; CD339; HJ1; JAGL1; protein jagged-1; jagged 1
Search name
Mouse Jag1 ELISA KIT ,Mouse JAGL1 ELISA KIT ,Mouse JAGGED1 ELISA KIT ,Mouse AGS ELISA KIT ,Mouse AHD ELISA KIT ,Mouse AWS ELISA KIT ,Mouse CD339 ELISA KIT ,Mouse HJ1 ELISA KIT ,Mouse protein jagged-1 ELISA KIT ,Mouse jagged 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse protein Jagged-1, Jagged1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Jagged1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Jagged1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Jagged1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
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