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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
A
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse A/G- specific adenine DNA glycosylase, Mutyh ELISA KIT
Product Name:Mouse A/G- specific adenine DNA glycosylase, Mutyh ELISA KIT
Packing:96T
Catalog No.:ELI-44785m
Gene Name:Mouse Mutyh
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Mutyh
Alternative Name:Mouse A/G-specific adenine DNA glycosylase, Mutyh
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse A/G- specific adenine DNA glycosylase, Mutyh ELISA KIT allows for the in vitro quantitative determination of Mouse Mutyh concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse A/G- specific adenine DNA glycosylase, Mutyh ELISA KIT has been pre-coated with an Mouse A/G-specific adenine DNA glycosylase, Mutyh antibody specific to Mouse Mutyh .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mutyh and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mutyh , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mutyh in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
) selected RNA are impossible to ignore. Figure 2. Differential Enrichment of Specific mRNAs by Oligo dT Chromatography. A Northern blot containing total RNA (1 µg) and twice oligo d(T) selected RNA (50 ng) from mouse
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
. Horton, J.R., Liebert, K., Hattman, S., Jeltsch, A., and Cheng, X. 2005. Transition from nonspecific to specific DNA interactions along the substrate‐recognition pathway of dam methyltransferase. Cell 121:349
in such cell death. By introducing human cDNA encoding a nuclear form of 8-oxoG DNA glycosylase (hOGG1-1a) into immortalized mouse embryo fibroblasts lacking Ogg1 gene, we established a cell line which selectively accumulates 8-oxoguanine in mitochondrial DNA
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