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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Origin recognition complex subunit 6, Orc6
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Origin recognition complex subunit 6, Orc6 ELISA KIT
Product Name:Mouse Origin recognition complex subunit 6, Orc6 ELISA KIT
Packing:96T
Catalog No.:ELI-15944m
Gene Name:Mouse Orc6
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Orc6
Alternative Name:Mouse Origin recognition complex subunit 6, Orc6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Origin recognition complex subunit 6, Orc6 ELISA KIT allows for the in vitro quantitative determination of Mouse Orc6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Origin recognition complex subunit 6, Orc6 ELISA KIT has been pre-coated with an Mouse Origin recognition complex subunit 6, Orc6 antibody specific to Mouse Orc6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Orc6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Orc6 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Orc6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验complex protein 6 (Orc6) (46) AAUGGUAGCCACAUCCGGUGU HeLa Origin recognition complex protein 6 (Orc6) (46) AAGATTGGACAGCAGGUCGAC HeLa P160 ROCK (47) 566-584; 639-657; 1958-1976; 2780-2798 See reference Polo-like Kinase1 (hPlk1) (37) NM_005030 181-203
Nup153 (1) NM_005124 2593-2615 aaggcagactctaccaaatgt Human HeLa View, Order View Origin recognition complex protein 6 (Orc6) (46) AAUGGUAGCCACAUCCGGUGU HeLa View, Order View Origin
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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