Mouse Secreted frizzled- related protein 3, Frzb ELISA KIT
Product Name:Mouse Secreted frizzled- related protein 3, Frzb ELISA KIT
Packing:96T
Catalog No.:ELI-15563m
Gene Name:Mouse Frzb
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Frzb
Alternative Name:Mouse Secreted frizzled-related protein 3, Frzb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Secreted frizzled- related protein 3, Frzb ELISA KIT allows for the in vitro quantitative determination of Mouse Frzb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Secreted frizzled- related protein 3, Frzb ELISA KIT has been pre-coated with an Mouse Secreted frizzled-related protein 3, Frzb antibody specific to Mouse Frzb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Frzb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Frzb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Frzb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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