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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Parathyroid hormone 2 receptor, Pth2r
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Parathyroid hormone 2 receptor, Pth2r ELISA KIT
Product Name:Mouse Parathyroid hormone 2 receptor, Pth2r ELISA KIT
Packing:96T
Catalog No.:ELI-15072m
Gene Name:Mouse Pth2r
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Pth2r
Alternative Name:Mouse Parathyroid hormone 2 receptor, Pth2r
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Parathyroid hormone 2 receptor, Pth2r ELISA KIT allows for the in vitro quantitative determination of Mouse Pth2r concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Parathyroid hormone 2 receptor, Pth2r ELISA KIT has been pre-coated with an Mouse Parathyroid hormone 2 receptor, Pth2r antibody specific to Mouse Pth2r .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pth2r and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pth2r , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pth2r in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity
are formed in response to various osteotropic factors such as 1α,25-dihydroxyvitamin D3 , parathyroid hormone, prostaglandin E2 , and interleukin -11. Recent studies have revealed that osteoblasts express two cytokines essential for osteoclastogenesis
Generating Murine Osteoclasts from Bone Marrow
to several bone-resorbing factors such as 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2 D3 ], parathyroid hormone (PTH), prostaglandin E2 (PGE2 ) and interleukin-11 (IL-11) (2 ,3 ). We also developed a mouse coculture system of primary osteoblasts and hemopoietic cells
G Protein-Coupled Receptors Disrupted in Human Genetic Disease
are discussed. Other receptors discussed include rhodopsin, thyrotropin, parathyroid hormone, melanocortin, follicle-stimulating hormone, luteinizing hormone, gonadotropin-releasing hormone (GnRHR), adren-ocorticotropic hormone, vasopressin, endothelin-β
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