- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14929m
- 2026年01月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
GTPase KRas, Kras
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse GTPase KRas, Kras ELISA KIT
Product Name:Mouse GTPase KRas, Kras ELISA KIT
Packing:96T
Catalog No.:ELI-14929m
Gene Name:Mouse Kras
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Kras
Alternative Name:Mouse GTPase KRas, Kras
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse GTPase KRas, Kras ELISA KIT allows for the in vitro quantitative determination of Mouse Kras concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse GTPase KRas, Kras ELISA KIT has been pre-coated with an Mouse GTPase KRas, Kras antibody specific to Mouse Kras .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kras and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kras , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kras in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验种（如 EGFR 突变、ALK/ROS1 重排、KRAS 突变、和 BRAF 突变等），利用基因编辑技术在小鼠上引入该突变基因，使小鼠自发肺癌表型，这类模型可用于肺癌发生与转移机制的研究，以及抗肿瘤药物的筛选和评价。下面小编将主要为大家介绍两种常见的自发肺癌模型。 KRAS 突变肺癌模型 KRAS 基因是首个被确定的原癌基因，其编码的基因是一种小的 GTP 水解酶，通过在激活（GTP 结合）和失活（GDP 结合）构象之间循环来控制多个信号级联通路，与 HRAS、NRAS 同属于 Ras 超蛋白
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
Cancer Cell：樊嘉 / 周虎等绘制肝内胆管癌多组学分子特征全景
53、KRAS、FGFR2、IDH1/2、BAP1 等肝内胆管癌主要驱动突变对蛋白质组和磷酸化蛋白质组的影响。研究发现，中国人群样本中特异性存在黄曲霉毒素突变指纹，与高肿瘤突变负荷和高 NK 细胞浸润等显著相关。FGFR2 的融合和突变可能通过激活 Rho GTPase 通路来促进 iCCA 发展，其部分融合蛋白衍生肽具有较强免疫原性，是潜在免疫抗原靶点。 随后，联合团队进一步分析了肝内胆管癌染色质拷贝数变异对 mRNA 及蛋白的顺式和反式调控效应。科研人员根据蛋白质组数据将 iCCA 患者分为炎症
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