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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Olfactory receptor 998, Olfr998
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Olfactory receptor 998, Olfr998 ELISA KIT
Product Name:Mouse Olfactory receptor 998, Olfr998 ELISA KIT
Packing:96T
Catalog No.:ELI-14900m
Gene Name:Mouse Olfr998
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Olfr998
Alternative Name:Mouse Olfactory receptor 998, Olfr998
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Olfactory receptor 998, Olfr998 ELISA KIT allows for the in vitro quantitative determination of Mouse Olfr998 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Olfactory receptor 998, Olfr998 ELISA KIT has been pre-coated with an Mouse Olfactory receptor 998, Olfr998 antibody specific to Mouse Olfr998 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Olfr998 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Olfr998 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Olfr998 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Raf 蛋白 Ras 结合结构域的简并进化库合成以及利用片段互补法快速筛选二氢叶酸还原酶的快速折叠且稳定的克隆
类似物, 其抑制大肠杆菌 DHFR 的能力是抑制哺乳动物 DHFR 的 12000 倍)处理的大肠杆菌的生长依赖于重组 mDHFR 的表达 [ 35 ] 。这样,将两个目的蛋白分别与 DHFR 的两个片段融合并共表达,如果两个蛋白质之间有相互作用,则大肠杆菌可以在添加了甲氧苄氨嘧啶的基本培养基上生长 [31]。在一个最初的文库间筛选中,DHFRPCA 被用于从含有 6 X1010 个可能结合的序列的单独文库中鉴定出可以形成最优的形成亮氨酸拉链的异源二聚体序列。竞争实验(competition
_003380 1037-1059 aaatggaagagaactttgccg Human HeLa vinculin, mouse (1) L18880 2923-2945 aagcggattagaaccaatctc Mouse 3T3 zyxin, mouse (1) X99063 1357-1379 aaagcctaccacccacagtgc Mouse 3T3 参考文献 Harborth J, Elbashir SM, Bechert K, Tuschl T
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