- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14374m
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
E3 ubiquitin-protein ligase RNF8, Rnf8
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse E3 ubiquitin- protein ligase RNF8, Rnf8 ELISA KIT
Product Name:Mouse E3 ubiquitin- protein ligase RNF8, Rnf8 ELISA KIT
Packing:96T
Catalog No.:ELI-14374m
Gene Name:Mouse Rnf8
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Rnf8
Alternative Name:Mouse E3 ubiquitin-protein ligase RNF8, Rnf8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse E3 ubiquitin- protein ligase RNF8, Rnf8 ELISA KIT allows for the in vitro quantitative determination of Mouse Rnf8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse E3 ubiquitin- protein ligase RNF8, Rnf8 ELISA KIT has been pre-coated with an Mouse E3 ubiquitin-protein ligase RNF8, Rnf8 antibody specific to Mouse Rnf8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rnf8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rnf8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rnf8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
【求助】E.coli修复DSB损伤与真核DSB方式有何不同？
nuclear factor family）成员确认为是DDR蛋白，它与γH2AX和MDC1一起发挥作用，应对细胞内出现的DSB。RNF8 包含一个 FHA 结构域，该结构域能和磷酸基苏氨酸（phospho-Thr）结合；RNF8的环指结构（RING finger）能与E3泛素连接酶作用。RNF8通过FHA结构域与 MDC1结合，不过这种结合作用依赖于MDC1的磷酸化作用。三个研究小组都一致发现在细胞发生DNA双链断裂现象时MDC1 会磷酸化，并对此做出应答。Kolas等人研究发现毛细血管扩张性共济失调突变
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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