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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Eif2s3y
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Eif2s3y ELISA KIT
Product Name:Mouse Eif2s3y ELISA KIT
Packing:96T
Catalog No.:ELI-14289m
Gene Name:Mouse Eif2s3y
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Eif2s3y
Alternative Name:Mouse Eif2s3y
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Eif2s3y ELISA KIT allows for the in vitro quantitative determination of Mouse Eif2s3y concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Eif2s3y ELISA KIT has been pre-coated with an Mouse Eif2s3y antibody specific to Mouse Eif2s3y .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eif2s3y and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eif2s3y , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eif2s3y in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验大鼠 神经肽Y (NP-Y ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中 神经肽Y (NP-Y ) 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中大鼠 神经肽 Y ( NP-Y ) 水平。用纯化的大鼠 神经肽 Y ( NP-Y ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 神经肽 Y ( NP
作为 ELISA 的最后一步,检测结果的计算对整个实验十分重要。 以夹心法为例。 1. ELISA 的样本通常要设置 1~2 个复孔进行检测,分别求出标准品、实验组样本的吸光度的平均值。单个样本的检测值不应超出平均值的 20%。 2. 样本的 OD(吸光度)平均值要减去标准品浓度为 0 时的 OD 平均值。 3. 建立标准曲线 在对数坐标图上拟合四参数的逻辑函数曲线(X 轴上为标准品浓度,Y 轴为对应的 OD 值)。推荐使用 origin 软件。 详细步骤如下: 第一步: 输入 OD 值
Characterization of Neuropeptide Y (NPY) Receptors
Gregor, P., Feng, Y., DeCarr, L.B., Cornfield, L.J., and McCaleb, M.L. 1996. Molecular characterization of a second mouse pancreatic polypeptide receptor and its inactivated human homologue. J. Biol. Chem. 271:27776‐27781
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