- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14284m
- 2026年01月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Prelamin-A
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Prelamin- A/C, LMNA ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LMNA,lamin A/C,CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; PRO1; 70 kDa lamin; lamin A/C-like 1; prelamin-A/C; renal carcinoma antigen NY-REN-32
Search name
Mouse LMNA ELISA KIT ,Mouse lamin A/C ELISA KIT ,Mouse CDCD1 ELISA KIT ,Mouse CDDC ELISA KIT ,Mouse CMD1A ELISA KIT ,Mouse CMT2B1 ELISA KIT ,Mouse EMD2 ELISA KIT ,Mouse FPL ELISA KIT ,Mouse FPLD ELISA KIT ,Mouse FPLD2 ELISA KIT ,Mouse HGPS ELISA KIT ,Mouse IDC ELISA KIT ,Mouse LDP1 ELISA KIT ,Mouse LFP ELISA KIT ,Mouse LGMD1B ELISA KIT ,Mouse LMN1 ELISA KIT ,Mouse LMNC ELISA KIT ,Mouse LMNL1 ELISA KIT ,Mouse PRO1 ELISA KIT ,Mouse 70 kDa lamin ELISA KIT ,Mouse lamin A/C-like 1 ELISA KIT ,Mouse prelamin-A/C ELISA KIT ,Mouse renal carcinoma antigen NY-REN-32 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofmousePrelamin-A/Cconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prelamin-A/C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forPrelamin-A/C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prelamin-A/C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Prelamin-A/C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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- 作者
- 内容
- 询问日期
文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
产生假阳性反应。一般说来，在 3 天内测定的血清标本可放置于 4℃，超过一周测定的需低温冰存。冻结血清融解后，蛋白质局部浓缩，分布不均，应充分混匀宜轻缓，避免气泡，可上下颠倒混和，不要在混匀器上强烈振荡。混浊或有沉淀的血清标本应先离心或过滤，澄清后再检测。反复冻融会使抗体效价跌落，所以测抗体的血清标本如需保存作多次检测，宜少量分装冰存。保存血清自采集时就应注意无菌操作，也可加入适当防腐剂。 2. 试剂的准备 按试剂盒说明书的要求准备实验中需用的试剂。 ELISA 中用的蒸馏水或去离子
Nature 解读：David R. Liu 等通过体内碱基编辑实现治疗小鼠Hutchinson-Gilford早衰综合征
Hutchinson-Gilford 早衰综合症（HGPS 或早衰症）通常是由编码核纤层蛋白 A 的基因 LMNA 中 C・G 基因突变为 T・A（c.1824 C> T；p.G608G）引起的。这种突变导致 RNA 错剪，产生早老素（progerin）。早老素是一种有毒蛋白，可引起快速衰老，导致早衰儿童的寿命大约只有 14 岁。尽管治疗早衰的策略例如全面抑制蛋白质法尼基化（farnesylation）可为患者带来益处，但尚没有报道可以直接逆转 HGPS 突变的方法。2021 年 1 月
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