- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14191m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
HMG box-containing protein 1, Hbp1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse HMG box- containing protein 1, Hbp1 ELISA KIT
Product Name:Mouse HMG box- containing protein 1, Hbp1 ELISA KIT
Packing:96T
Catalog No.:ELI-14191m
Gene Name:Mouse Hbp1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Hbp1
Alternative Name:Mouse HMG box-containing protein 1, Hbp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse HMG box- containing protein 1, Hbp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Hbp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse HMG box- containing protein 1, Hbp1 ELISA KIT has been pre-coated with an Mouse HMG box-containing protein 1, Hbp1 antibody specific to Mouse Hbp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hbp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hbp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hbp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Alternate Protocol 1: RNA Preparation from Pure RPE Cells Basic Protocol 4: Protein Preparation from Eye Tissues Basic Protocol 5: Fixation, Inclusion, and Cryosection of the Mouse Eye
are done as follows: Cover with plate sealer tape or place in a sealed box containing a wet paper towel and incubate for 2 hours at room temperature. For the four controls: 2 are negative controls using pre-bleeds of the same concentration as your staring dilution
Eccles:Protein Lysates from Tissue
Cell Lysis Buffer containing 1x Complete protease inhibitor (for embryonic mouse kidney 100uL is suitable) Crush tissue using pestle until tissue is completely pulverised taking care to keep it cold Incubate on ice for 5-10min
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