Core histone macro-H2A.2, H2af
y2 ELISA KIT/ Mouse Core histone macro-H2A.2, H2afy2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14180m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Core histone macro-H2A.2, H2afy2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Core histone macro- H2A.2, H2afy2 ELISA KIT
Product Name:Mouse Core histone macro- H2A.2, H2afy2 ELISA KIT
Packing:96T
Catalog No.:ELI-14180m
Gene Name:Mouse H2afy2
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse H2afy2
Alternative Name:Mouse Core histone macro-H2A.2, H2afy2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Core histone macro- H2A.2, H2afy2 ELISA KIT allows for the in vitro quantitative determination of Mouse H2afy2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Core histone macro- H2A.2, H2afy2 ELISA KIT has been pre-coated with an Mouse Core histone macro-H2A.2, H2afy2 antibody specific to Mouse H2afy2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse H2afy2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse H2afy2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse H2afy2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Examining Histone Acetlylation at Specific Genomic Regions
The acetylation of core histones can modulate the expression of numerous genes. In general, the deacetylation of histones results in transcriptional repression, whereas increases in histone acetylation lead to the enhancement of gene
tag on either the sense or antisense strand (City of Hope Protein and synthesis core facility) and various iterations were generated by first heating the RNAs (sense or antisense +/- biotin) to 70°C for 5 minutes and then mixed together and room
, and lysine ubiquitination. On their own, or in combination, these covalent modifications on the core histones are thought to play essential roles in chromatin organization and gene expression in eukaryotes. Importantly, patterns of histone modifications
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