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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Histone H2A type 1-H, Hist1h2ah
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Histone H2A type 1- H, Hist1h2ah ELISA KIT
Product Name:Mouse Histone H2A type 1- H, Hist1h2ah ELISA KIT
Packing:96T
Catalog No.:ELI-14179m
Gene Name:Mouse Hist1h2ah
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Hist1h2ah
Alternative Name:Mouse Histone H2A type 1-H, Hist1h2ah
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histone H2A type 1- H, Hist1h2ah ELISA KIT allows for the in vitro quantitative determination of Mouse Hist1h2ah concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Histone H2A type 1- H, Hist1h2ah ELISA KIT has been pre-coated with an Mouse Histone H2A type 1-H, Hist1h2ah antibody specific to Mouse Hist1h2ah .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hist1h2ah and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hist1h2ah , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hist1h2ah in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, for example, this protocol yields ~2 x 109 nuclei/g tissue. Nuclei Preparation from Cultured Cells (2 h) 9. Culture 1 x 107 to 1 x 108 cells. Ensure that the cells are not grown beyond semiconfluency. 10. Rinse
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
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