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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Raet1d
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Raet1d ELISA KIT
Product Name:Mouse Raet1d ELISA KIT
Packing:96T
Catalog No.:ELI-13996m
Gene Name:Mouse Raet1d
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Raet1d
Alternative Name:Mouse Raet1d
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Raet1d ELISA KIT allows for the in vitro quantitative determination of Mouse Raet1d concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Raet1d ELISA KIT has been pre-coated with an Mouse Raet1d antibody specific to Mouse Raet1d .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Raet1d and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Raet1d , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Raet1d in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验膜蛋白依附或横跨在细胞膜的磷脂双分子层上,使膜蛋白的表达、纯化、结晶和结构分析都有很大难度。由于非常难以获得高纯度的膜蛋白,进而解析它们的结构和功能,使根据膜蛋白结构设计药物的方法难以施展,而这种方法是目前开发药物最有效的方法之一,因为药物要能有效治疗并且使副作用最小化,作用位点就必须精准。很多科研人员想深入研究膜蛋白,希望攻克困扰人类的各种疾病,但由于提取高纯度目标膜蛋白并不便捷,从而影响研发进度。目前市面上有一些专用于膜蛋白提取的试剂盒,大多数只能提取某几种类型的膜蛋白, 有些试剂盒只能提取膜
and some B cell malignancies Negative staining: normal B cells CD1d Positive staining (normal): thymus (low levels), bowel ELISA KIT & ANTIBODY OF USCNLIFE
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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